http://www.omicsgroup.org/journals/cloning-transgenesis.php

Category: Academics

Gene cloning (DNA cloning) Gene cloning (DNA cloning) is a genetic engineering technique that promotes the production of exact copies of a spoecific DNA sequence. In Gene cloning the DNA with a target gene(s) is split into fragments using restriction enzymes and then inserted into cloning vectors like plasmids which transfer the recombinant DNA to suitable host cells, such as the bacterium E. coli. Plasmid vector Plasmid vector is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. Plasmid vectors are most commonly found in bacteria as small, circular, double-stranded DNA molecules.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host.